WB
Western blot analysis using OCT-4 antibody against Hela(1),Hepg2(2),Mcf-7(3),A549(4),Cos7(5),A431(6),K562(7) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using OCT-4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using OCT-4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using OCT-4 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using OCT-4 antibody with DAB staining.| Product Name | Anti-OCT-4 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POU5F1 |
|---|---|
| Gene Synonyms | OCT3 OCT4 OTF3 OTF4 OTF-3 Oct-3 Oct-4 |
| Gene Full Name | POU class 5 homeobox 1 |
| Gene Summary | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] |
| Molecular Weight(MW) | 39 kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q01860 |
|---|
WB
Western blot analysis using OCT-4 antibody against Hela(1),Hepg2(2),Mcf-7(3),A549(4),Cos7(5),A431(6),K562(7) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using OCT-4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using OCT-4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using OCT-4 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using OCT-4 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
