ICC/IF
Immunofluorescence analysis of NIH3T3 cells using CD4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CD4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD4 |
|---|---|
| Gene Synonyms | CD3-DELTA CD3-GAMMA CD3-ZETA CD3DELTA CD3epsilon CD3GAMMA CD3H CD3Q CD3Z CD3ZETA CD4mut IMD17 IMD18 IMD19 IMD25 IMD79 Leu-3 OKT4D T3D T3E T3G T3Z T4 TCRE TCRZ |
| Gene Full Name | CD4 molecule |
| Gene Summary | This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
| Alternative Names | CD247 CD247 antigen zeta subunit CD3 antigen delta subunit CD3 delta CD3-epsilon CD3d antigen delta polypeptide (TiT3 complex) CD3d molecule delta (CD3-TCR complex) CD3e antigen epsilon polypeptide (TiT3 complex) CD3e molecule epsilon (CD3-TCR complex) CD3g antigen gamma polypeptide (TiT3 complex) CD3g molecule epsilon (CD3-TCR complex) CD3g molecule gamma (CD3-TCR complex) CD3Z antigen zeta polypeptide (TiT3 complex) CD3zeta chain CD4 antigen (p55) CD4 antigen p55 CD4 receptor component of the TCR/CD3 complex FLJ18683 Leu-2 MAL OKT3 delta chain OKT8 T-cell antigen T cell co-receptor T-cell antigen Leu2 T-cell antigen receptor complex epsilon subunit of T3 T-cell antigen receptor complex gamma subunit of T3 T-cell antigen receptor complex zeta subunit of CD3 T-cell receptor T3 delta chain T-cell receptor T3 gamma chain T-cell receptor T3 zeta chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface antigen T4/Leu-3 T4 surface glycoprotein precursor T8 T-cell antigen TCR zeta TCR zeta chain tissue factor-targeting CAR1 dimer tissue factor-targeting CAR1 monomer |
| Molecular Weight(MW) | 51 kDa |
| Cellular Localization | Plasma membrane |
| SwissProt ID | P01730 |
|---|
ICC/IF
Immunofluorescence analysis of NIH3T3 cells using CD4 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 20 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
