WB
Loading:1 μg of total protein from Arabidopsis thaliana/rice leaves Antigen proteins with gradient dilution Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-MDH / Malate Dehydrogenase rabbit antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana At3g47520. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | MDH |
|---|---|
| Gene Synonyms | MDH, Malate Dehydrogenase |
| Gene Full Name | MDH / Malate Dehydrogenase rabbit antibody |
| Gene Summary | Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments. Plays a key role in the metabolism of dark chloroplasts and non-green plastids. Essential for embryo viability (PubMed:24198233, PubMed:24453164). Plays an essential role in heterotrophic metabolism in embryos, and autotrophic metabolism in photosynthetic tissues as well (PubMed:24453164). |
| Molecular Weight(MW) | 42 / 33 kDa |
WB
Loading:1 μg of total protein from Arabidopsis thaliana/rice leaves Antigen proteins with gradient dilution Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
