WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-MEKK1/Mitogen-activated protein kinase kinase kinase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana At4g08500 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | MEKK1 |
|---|---|
| Gene Synonyms | ARAKIN, AtMEKK1, MAP kinase kinase kinase 1, MAPKKK8 |
| Gene Full Name | MEKK1/Mitogen-activated protein kinase kinase kinase 1 |
| Gene Summary | The MEKK (MAPK/ERK kinase kinase) family has four members: At4g08500 (MEKK1), At4g08480 (MEKK2), At4g08470 (MEKK3) and At4g12020 (MEKK4). They function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses, and also play an important role in pathogen defense by negatively regulating innate immunity. |
| Molecular Weight(MW) | 66 kDa |
WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
