WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-NIR1/ Ferredoxin--nitrite reductase, chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT2G15620，Q39161 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | NIR1 |
|---|---|
| Gene Synonyms | ARABIDOPSIS THALIANA NITRITE REDUCTASE, ATHNIR, NIR, NIR1, NITRITE REDUCTASE, NITRITE REDUCTASE 1 |
| Gene Full Name | NIR1/ Ferredoxin--nitrite reductase, chloroplastic |
| Gene Summary | NIR1 catalyzes the six-electron reduction of nitrite to ammonium. It is involved in the second step of nitrate assimilation. Its expression is induced by nitrate. |
| Molecular Weight(MW) | 65.5 / 63 kDa |
WB
Loading:Approximately 3 μg of total proteins from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
