WB
Loading:Antigen proteins with gradient dilution Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-PEN3/ ABC transporter G family member 36 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G59870，Q9XIE2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | PEN3 |
|---|---|
| Gene Synonyms | ABCG36，PDR8，PEN3，ABC transporter ABCG.36，Protein PENETRATION 3，Pleiotropic drug resistance protein 8 |
| Gene Full Name | PEN3/ ABC transporter G family member 36 |
| Gene Summary | ATP binding cassette transporter. Localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated at infection sites. Together with ABCG37, ABCG36 regulates auxin homeostasis and responses by playing a dual role in coumarine (e.g. esculin) and in the auxin precursor indole 3-butyric acid (IBA) efflux transport, thus influencing cotyledons, roots and root hairs development. |
| Molecular Weight(MW) | 165 kDa |
WB
Loading:Antigen proteins with gradient dilution Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
