WB
Loading:1 μg of total protein from Arabidopsis thaliana/rice/soybean leaves
Electrophoresis: 12% SDS-PAGE
Membrane transfer: NC (nitrocellulose) membrane, 10V overnight
Blocking: 5% skim milk, incubation at room temperature for 1h
Primary antibody: 1:5000 dilution, incubation at room temperature for 1h