WB
Loading:Antigen proteins with gradient dilution Electrophoresis: 10% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-RBOHD/ Respiratory burst oxidase homolog protein D |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT5G47910，Q9FIJ0 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | RBOHD |
|---|---|
| Gene Synonyms | ATRBOHD, DELT1, RBOHD, RESPIRATORY BURST OXIDASE HOMOLOGUE D |
| Gene Full Name | RBOHD/ Respiratory burst oxidase homolog protein D |
| Gene Summary | RBOHD is a calcium-dependent NADPH oxidase that generates superoxide. It is involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens. |
| Molecular Weight(MW) | 104 kDa |
WB
Loading:Antigen proteins with gradient dilution Electrophoresis: 10% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
