WB
Western blot analysis using PPP2R1A antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | PPP2R1A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPP2R1A |
|---|---|
| Gene Synonyms | 6330556D22Rik HJS2 MRD36 PP2A PP2A-Aalpha PP2AA PP2AAALPHA PR65 PR65A |
| Gene Full Name | protein phosphatase 2 scaffold subunit Aalpha |
| Gene Summary | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
| Alternative Names | medium tumor antigen-associated 61 KDA protein MGC786 PP2Ac PP2CA PP2Calpha PPP2R1A protein phosphatase 2 regulatory subunit A alpha protein phosphatase PP2A RP-C serine/threonine protein phosphatase A subunit type 2A testicular secretory protein Li 1 |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cytoplasm;Nucleus;Chromosome;Chromosome, centromere;Lateral cell membrane;Cell projection, dendrite |
| SwissProt ID | P30153 |
|---|
WB
Western blot analysis using PPP2R1A antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
