WB
Western blot analysis using PRKACA antibody against Hepg2(1),C2Cl2(2),Cos-7(3),Nih3t3(3),Mcf-7(4),Hek293(5),B16(6),P19(7) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using PRKACA antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PRKACA antibody with DAB staining.| Product Name | Anti-PRKACA antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKACA |
|---|---|
| Gene Synonyms | CAFD1 PKACA PPNAD4 |
| Gene Full Name | protein kinase cAMP-activated catalytic subunit alpha |
| Gene Summary | This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015] |
| Molecular Weight(MW) | 41kDa |
| Cellular Localization | Cell membrane, Cell projection, Cilium, Cytoplasm, Cytoplasmic vesicle, Flagellum, Membrane, Mitochondrion, Nucleus |
| SwissProt ID | P17612 |
|---|
WB
Western blot analysis using PRKACA antibody against Hepg2(1),C2Cl2(2),Cos-7(3),Nih3t3(3),Mcf-7(4),Hek293(5),B16(6),P19(7) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using PRKACA antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PRKACA antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
