WB
Western blot analysis using PI 3 Kinase p55 antibody against Huvec(1),P19(2),C2C12(3),B16(4),Hek293(5),K562(6) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ICC/IF
Immunofluorescence analysis of HUVEC cells using PI 3 Kinase p55 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-PI 3 Kinase p55 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000-1:2000 ICC/IF:1:100-1:500ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PIK3R3 |
|---|---|
| Gene Synonyms | AGM7 GRB1 IMD36 MPPH MPPH1 p50alpha p55 p55-GAMMA p55alpha p55PIK p85 p85-ALPHA p85-BETA p85alpha P85B p85beta PI3K PI3KA |
| Gene Full Name | phosphoinositide-3-kinase regulatory subunit 3 |
| Gene Summary | Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016] |
| Alternative Names | growth factor receptor bound 1 OTTHUMP00000009783 OTTHUMP00000009784 p55PIK p85-alpha GRB1 phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 (p55) phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta phosphatidylinositol 3-kinase p55 subunit phosphatidylinositol 3-kinase p85 alpha phosphatidylinositol 3-kinase regulartory subunit polypeptide 2 phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphatidylinositol 3-kinase regulatory subunit polypeptide 2 (p85 beta) phosphatidylinositol 3-kinase regulatory subunit polypeptide 3 (p55 gamma) phosphatidylinositol 3-kinase-associated p-85 alpha Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50) Phosphoinositide 3-kinase regulatory subunit polypeptide 1 (p85 alpha) phosphoinositide-3-kinase regulatory subunit alpha phosphoinositide-3-kinase regulatory subunit beta phosphoinositide-3-kinase regulatory subunit 1 (alpha) phosphoinositide-3-kinase regulatory subunit 2 (beta) phosphoinositide-3-kinase regulatory subunit 3 (gamma) PI3 kinase-associated p85 PI3-kinase p85 subunit alpha PI3-kinase p85 subunit beta PI3-kinase p85 subunit gamma PI3-kinase regulatory subunit alpha PI3-kinase regulatory subunit beta PI3-kinase regulatory subunit gamma PI3-kinase subunit p55-gamma PI3-kinase subunit p85-alpha PI3-kinase subunit p85-beta PI3K regulatory subunit alpha PI3K regulatory subunit beta PI3K regulatory subunit gamma PI3K-p46 polypeptide 3 ptdIns-3-kinase p85-alpha ptdIns-3-kinase p85-beta ptdIns-3-kinase p85-gamma ptdIns-3-kinase regulatory subunit alpha ptdIns-3-kinase regulatory subunit beta ptdIns-3-kinase regulatory subunit gamma ptdIns-3-kinase regulatory subunit p55-gamma PtdIns-3-kinase regulatory subunit p85-al ptdIns-3-kinase regulatory subunit p85-alpha ptdIns-3-kinase regulatory subunit p85-beta RP4-533D7.2 |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Cytosol |
| SwissProt ID | Q92569 |
|---|
WB
Western blot analysis using PI 3 Kinase p55 antibody against Huvec(1),P19(2),C2C12(3),B16(4),Hek293(5),K562(6) cell lysate. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ICC/IF
Immunofluorescence analysis of HUVEC cells using PI 3 Kinase p55 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:100-1:500ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
