WB
Western blot analysis using CYP1A1 antibody against Rat stomach(1),Rat spleen(2) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-CYP1A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CYP1A1 |
|---|---|
| Gene Synonyms | AHH AHRR CP11 CYP1 Cyp45c Cypc45c CYPIA1 P-450MC P1-450 P450-1 P450-C P450DX P450form6 P450MT2 |
| Gene Full Name | cytochrome P450 family 1 subfamily A member 1 |
| Gene Summary | This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2016] |
| Alternative Names | aryl hydrocarbon hydroxylase CYP CYP1A1v alternatively spliced cytochrome P-450 protein cytochrome P-450-1 cytochrome P1 cytochrome P1-450 cytochrome P1-450 dioxin-inducible cytochrome P450 form 6 cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c) cytochrome P450 subfamily I polypeptide 1 cytochrome P450 1a1 cytochrome P450 1a1 aromatic compound inducible cytochrome P450 family 1 subfamily A polypeptide 1 cytochrome P450 subfamily I (aromatic compound-inducible) member A1 (C6 form c) (C6 form c) cytochrome P450 subfamily I (aromatic compound-inducible) polypeptide 1 cytochrome P450-C cytochrome P450-P1 cytochrome P450MT2 dioxin-inducible flavoprotein-linked monooxygenase functional drug metabolizing enzyme hydroperoxy icosatetraenoate dehydratase microsomal monooxygenase P450 P450 form 6 P450-P1 xenobiotic monooxygenase |
| Molecular Weight(MW) | 58KD |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membrane |
| SwissProt ID | P04798 |
|---|
WB
Western blot analysis using CYP1A1 antibody against Rat stomach(1),Rat spleen(2) tissue. 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
