WB
Western blot analysis using Beta actin mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S| Product Name | Anti-Beta actin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:1000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Actb |
|---|---|
| Gene Synonyms | actb actin Actx Bact Beta-actin BKRNS BNS BRWS1 CSMH DDS1 E430023M04Rik PS1TP5BP1 THC8 XELAEV_18045052mg |
| Gene Full Name | actin, beta |
| Gene Summary | This gene encodes a member of the actin family of proteins. Actins are highly conserved proteins that are among the most abundant proteins in eukaryotic cells and are involved in cell motility, structure, and integrity. Localization, stability, and translation of the transcribed mRNA are regulated through the binding of multiple factors to its 3' UTR sequence. Homozygous knockout mice for this gene exhibit embryonic lethality. Numerous pseudogenes of this gene have been identified in the mouse genome. [provided by RefSeq, Sep 2015] |
| Alternative Names | A-X actin A-X actin-like protein Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments which subsequently assemble into interconnected networks within the cytoplasm of cells. actin beta cytoplasmic Bact actin beta actin beta cytoskeletal actin beta-actin cytoplasmic 1 cytoplasmic beta-actin cytoplasmic protein cytoskeletal beta actin I(2)-actin melanoma X-actin PS1TP5-binding protein 1 |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cytoplasm Membrane Nucleus |
| SwissProt ID | P60709 |
|---|
WB
Western blot analysis using Beta actin mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S| Application Notes | WB:1:1000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
