WB
Western blot analysis using PARP mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SICC/IF
Immunofluorescence analysis of Hela cells using PARP antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Mouse IgG(H&L) (Green,OM643485), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-PARP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:1000 ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARP |
|---|---|
| Gene Synonyms | 5830444G22Rik Adprp ADPRT ADPRT 1 ADPRT1 ARTD1 msPARP pADPRT-1 PARP PARP-1 PARS Poly-PARP PPOL sPARP-1 |
| Gene Full Name | poly(ADP-ribose) polymerase |
| Gene Summary | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008] |
| Alternative Names | ADP-ribosyltransferase (NAD+ ADP-ribosyltransferase (NAD+) poly (ADP-ribose) polymerase) ADP-ribosyltransferase (NAD+ poly (ADP-ribose) polymerase) 1 ADP-ribosyltransferase (NAD+ poly (ADP-ribose) polymerase) ADP-ribosyltransferase 1 ADP-ribosyltransferase diphtheria toxin-like 1 ADP-ribosyltransferase NAD(+) ADPRT 1 DNA ADP-ribosyltransferase PARP1 NAD(+) ADP-ribosyltransferase 1 PARP-1 poly (ADP-ribose) polymerase family member 1 poly (ADP-ribose) polymerase) poly(ADP-ribose) polymerase poly(ADP-ribose) polymerase PARP-1 poly(ADP-ribose) synthetase poly(ADP-ribosyl)transferase poly[ADP-ribose] synthase 1 poly[ADP-ribose] synthetase 1 protein poly-ADP-ribosyltransferase PARP1 |
| Molecular Weight(MW) | 116kDa |
| Cellular Localization | Chromosome Nucleus |
| SwissProt ID | P09874 |
|---|
WB
Western blot analysis using PARP mouse monoclonal antibody against Hela(1),Hepg2(2),A549(3),Huvec(4),Cos-7(5),P19(6) cell lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ICC/IF
Immunofluorescence analysis of Hela cells using PARP antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Mouse IgG(H&L) (Green,OM643485), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000 ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
