WB
Western blot analysis using GAP43 antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2(6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-GAP43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | GAP43 |
|---|---|
| Gene Synonyms | B-50 Basp2 GAP-43 PP46 |
| Gene Full Name | growth associated protein 43 |
| Gene Summary | The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | axonal membrane protein GAP-43 brain abundant membrane attached signal protein 2 calmodulin-binding protein calmodulin-binding protein P-57 GAP-43 protein growth accentuating protein 43 growth-associated protein 43 MGC156666 nerve growth-related peptide GAP43 neural phosphoprotein B-50 neuron growth-associated protein 43 neuron protein F1 neuronal growth factor 43 protein F1 |
| Molecular Weight(MW) | 25kDa（observed：43kDa） |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Membrane,Synapse. |
| SwissProt ID | P17677 |
|---|
WB
Western blot analysis using GAP43 antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2(6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
