WB
Western blot analysis using Histone H2B antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2(6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 3SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-Histone H2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | H2BC3 |
|---|---|
| Gene Synonyms | H2b-143 H2B.1 H2B/f H2BFF HIST1H2BB |
| Gene Full Name | H2B clustered histone 3 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Alternative Names | H2B histone family member F H2BFF HIST1H2BB histone 1 H2bb histone cluster 1 H2B family member b histone cluster 1 H2bb histone H2B.1 histone H2B.f |
| Molecular Weight(MW) | 14kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P33778 |
|---|
WB
Western blot analysis using Histone H2B antibody against Huvec(1),Thp-1(2),Nih3t3(3),Mcf-7(4),Hela(5),Hepg2(6),A549(7),Hek293(8),Jurkat(9),C2C12(10),Cos-7(11),Pc-12(12),K562(13),A431(14)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 3S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
