WB
Western blot analysis using Drosophila Melanogaster GAPDH antibody against 3rd instar larva(1)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60SELISA
Blue line: Antibody; Orange line: Isotype control antibody| Product Name | Anti-Drosophila Melanogaster GAPDH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | Drosophila |
| Purification | Affinity purified |
| Gene Symbol | Gpdh1 |
|---|---|
| Gene Synonyms | alpha-GPD alpha-Gpdh alpha-GPDH alpha-GPDH-1 alphaGpd alphaGpdh alphaGPDH alphaGpdh-1 CG9042 dGpdh Dmel\CG9042 DmG3PDH DROGPDHA G3pdh G3PDH GAPDH Gdh Gpd GPD GPDA gpdh Gpdh GPDH gpdh-1 GPDH-1 GPDH1 sn-Gpdh |
| Gene Full Name | Glycerol-3-phosphate dehydrogenase 1 |
| Gene Summary | Enables glycerol-3-phosphate dehydrogenase (NAD+) activity. Involved in several processes, including flight behavior; glycerol-3-phosphate biosynthetic process; and nicotinamide nucleotide metabolic process. Located in M band; Z disc; and cytosol. Is expressed in adult head; adult heart; and flight muscle cell. Human ortholog(s) of this gene implicated in Brugada syndrome 2. Orthologous to several human genes including GPD1 (glycerol-3-phosphate dehydrogenase 1). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 38 kDa BFA-dependent ADP-ribosylation substrate antibody aging associated gene 9 protein antibody Aging-associated gene 9 protein antibody BARS-38 antibody cb609 antibody EC 1.2.1.12 antibody Epididymis secretory sperm binding protein Li 162eP antibody G3P_HUMAN antibody G3PD antibody G3PDH antibody |
| Molecular Weight(MW) | 38kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
| SwissProt ID | P04406 |
|---|
WB
Western blot analysis using Drosophila Melanogaster GAPDH antibody against 3rd instar larva(1)lysate . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Mouse IgG (H&L) - HRP（OM644366）, at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S
ELISA
Blue line: Antibody; Orange line: Isotype control antibody| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide,40% Glycerol. |
