WB
Western blot analysis using PRAME antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13),Raji(14) cell .WB
Western blot analysis using PRAME antibody against Mouse lung tissue(1),Mouse spleen tissue(2) .IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PRAME antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PRAME antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using PRAME antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using PRAME antibody with DAB staining.| Product Name | Anti-PRAME antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRAME |
|---|---|
| Gene Synonyms | MAPE OIP4 CT130 OIP-4 |
| Gene Full Name | PRAME nuclear receptor transcriptional regulator |
| Gene Summary | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Molecular Weight(MW) | 58kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus,Cell membrane,Cytoplasm |
| SwissProt ID | P78395 |
|---|
WB
Western blot analysis using PRAME antibody against Hela(1),Hepg2(2),K562(3),A549(4),A431(5),Huvec(6),Nih3t3(7),Cos-7(8),C2C12(9),Hek293(10),Mcf-7(11),Pc-12(12),Jurkat(13),Raji(14) cell .
WB
Western blot analysis using PRAME antibody against Mouse lung tissue(1),Mouse spleen tissue(2) .
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using PRAME antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PRAME antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using PRAME antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using PRAME antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
