WB
K-562 cells were subjected to SDS PAGE followed by western blot with PRAME antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IP
IP result of anti-PRAME (4ug; Detection:1:300) with K-562 cells lysate 2400 ug.IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using PRAME antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Product Name | PRAME Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PRAME protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:1000 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRAME |
|---|---|
| Gene Synonyms | MAPE OIP4 CT130 OIP-4 |
| Gene Full Name | PRAME nuclear receptor transcriptional regulator |
| Gene Summary | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Alternative Names | MAPE OIP 4 OIP4 OIP-4 Opa-interacting protein 4 |
| Molecular Weight(MW) | 58 kDa(Observed Molecular Weight:50-58 kDa) |
| Cellular Localization | Cell membrane,Chromosome,Cytoplasm,Golgi apparatus,Membrane,Nucleus. |
WB
K-562 cells were subjected to SDS PAGE followed by western blot with PRAME antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP
IP result of anti-PRAME (4ug; Detection:1:300) with K-562 cells lysate 2400 ug.
IHC
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using PRAME antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).| Application Notes | WB:1:500-1:1000 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
