WB
Various lysates were subjected to SDS PAGE followed by western blot with PRAME antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.IHC
Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti Mouse IgG(H+L), CL594-phalloidin (red).| Product Name | PRAME Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human PRAME protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| WB:1:5000-1:50000 ICC: 1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRAME |
|---|---|
| Gene Synonyms | MAPE OIP4 CT130 OIP-4 |
| Gene Full Name | PRAME nuclear receptor transcriptional regulator |
| Gene Summary | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Alternative Names | MAPE OIP 4 OIP4 Opa interacting protein 4 PRAME |
| Molecular Weight(MW) | 58kDa( Observed MW: 50kDa) |
| Cellular Localization | Nucleus,Cytoplasm,Cell membrane. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with PRAME antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC
Immunofluorescent analysis of (4% PFA) fixed HaCaT cells using PRAME antibody at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti Mouse IgG(H+L), CL594-phalloidin (red).| Application Notes | WB:1:5000-1:50000 ICC: 1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
