ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of A549 cells using CDK11Bantibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-CDK11B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDK11B |
|---|---|
| Gene Synonyms | Cdc11b CDC2L1 CDC2L2 CDC2L3 CDK11 CDK11-p110 CDK11-p46 CDK11-p58 CLK-1 p58 p58CDC2L1 p58CLK-1 p58GTA PITSLRE PITSLREA PK58 |
| Gene Full Name | cyclin dependent kinase 11B |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| Alternative Names | CDC-related protein kinase p58 cell division cycle 2 homolog-like 1 cell division cycle 2 homolog-like 2 cell division cycle 2-like 1 cell division cycle 2-like 1 (PITSLRE proteins) cell division cycle 2-like 2 cell division cycle 2-like 2 (PITSLRE proteins) cell division cycle 2-like protein kinase 1 cell division cycle 2-like protein kinase 2 cell division protein kinase 11 cell division protein kinase 11A cell division protein kinase 11B cyclin-dependent kinase 11 galactosyltransferase-associated protein kinase p58/GTA GR specific protein kinase p58 CLK-1 PITSLRE B PITSLRE proteins PITSLRE serine/threonine-protein kinase CDC2L1 PITSLRE serine/threonine-protein kinase CDC2L2 |
| Molecular Weight(MW) | 92kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus;Cytoplasm |
| SwissProt ID | P21127 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of A549 cells using CDK11Bantibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
