ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using GNAO1 antibody against C2C12(1),P19(2),Huvec(3),Mcf-7(4),A431(5),Pc-12(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-GNAO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GNAO1 |
|---|---|
| Gene Synonyms | alphaO DEE17 EIEE17 G-ALPHA-o Galphao GNAO HG1G NEDIM RATBPGTPC |
| Gene Full Name | G protein subunit alpha o1 |
| Gene Summary | The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
| Alternative Names | DKFZp686O0962 G alpha o G protein GNAO 1 Go alpha Go alpha-1a subunit splice variant Go alpha-1b subunit splice variant GO2-q chimeric G-protein GTP-binding protein GTP-binding protein alpha o GTP-binding protein alpha subunit (G0A-alpha) GTP-binding protein alpha subunit (G0B-alpha) GTP-binding protein alpha-o subunit guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O guanine nucleotide binding protein alpha O polypeptide 1 Guanine nucleotide-binding protein alpha subunit guanine nucleotide-binding regulatory protein 2 heterotrimeric guanine nucleotide-binding protein 1G |
| Molecular Weight(MW) | 40kDa（observed：37kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane |
| SwissProt ID | P09471 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using GNAO1 antibody against C2C12(1),P19(2),Huvec(3),Mcf-7(4),A431(5),Pc-12(6) cell . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
