ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.ICC/IF
Immunofluorescence analysis of U251 cells using KCNH2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Product Name | Anti-KCNH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KCNH2 |
|---|---|
| Gene Synonyms | cerg derg ERG ERG-1 ERG1 H-ERG HERG HERG1 I79_007694 Kv11.1 LQT LQT2 M-erg Merg1 merg1a merg1b SQT1 |
| Gene Full Name | potassium voltage-gated channel subfamily H member 2 |
| Gene Summary | This gene encodes a component of a voltage-activated potassium channel found in cardiac muscle, nerve cells, and microglia. Four copies of this protein interact with one copy of the KCNE2 protein to form a functional potassium channel. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, May 2022] |
| Alternative Names | c-ERG cERG ether-a-go-go related member 2 delayed rectifier potassium channel eag homolog eag related protein 1 eag-related gene eag-related protein 1 ERG-1 ERG1 ether-a-go-go related gene potassium channel 1 ether-a-go-go related protein Ether-a-go-go-related gene potassium channel 1 ether-a-go-go-related potassium channel protein ether-a-go-go-related protein ether-a-go-go-related protein 1 HERG-1 human ether-a-go-go-related long QT syndrome type 2 M-erg1 potassium channel potassium channel protein ERG potassium channel voltage gated eag related subfamily H member 2 Potassium voltage-gated channel subfamily H member 2 potassium voltage-gated channel subfamily H (eag-related) member 2 r-ERG ra-erg RERG subfamily H eag-related ventricular ERG K+ channel subunit voltage-gated potassium channel 11.1 voltage-gated potassium channel subunit Kv11.1 voltage-gated potassium channel subfamily H member 2 |
| Molecular Weight(MW) | 126kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q12809 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
ICC/IF
Immunofluorescence analysis of U251 cells using KCNH2 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.| Application Notes | ICC/IF:1:100-1:500 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
