WB
Western blot analysis of lysates from COLO205 cells(LPS,4h treatment), using KCNH2 Ab at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 40s with ECL Kit. Bands result from membrane strip incubation.WB
Western blot analysis of lysates from Mouse testis, using KCNH2 Antibody at 1/1000 dilution.IHC
Rabbit polyclonal antibody to KCNH2 at 1/100 staining human astrocytoma by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.ICC/IF
Rabbit polyclonal antibody to KCNH2 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to KCNH2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human KCNH2, corresponding to a region within C-terminal amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNH2 |
|---|---|
| Gene Synonyms | ERG1 HERG LQT2 SQT1 ERG-1 H-ERG HERG1 Kv11.1 |
| Gene Full Name | potassium voltage-gated channel subfamily H member 2 |
| Gene Summary | This gene encodes a component of a voltage-activated potassium channel found in cardiac muscle, nerve cells, and microglia. Four copies of this protein interact with one copy of the KCNE2 protein to form a functional potassium channel. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, May 2022] |
| Alternative Names | eag homolog Eag-related protein 1 ERG ERG-1 ERG1 Ether a go go related potassium channel protein Ether-a-go-go-related gene potassium channel 1 Ether-a-go-go-related protein 1 H ERG H-ERG HERG 1 HERG hERG-1 hERG1 KCNH2 KCNH2_HUMAN Kv11.1 LQT 2 LQT2 Potassium channel HERG Potassium voltage gated channel subfamily H (eag related) member 2 Potassium voltage-gated channel subfamily H member 2 SQT1 Voltage gated potassium channel subfamily H member 2 Voltage-gated potassium channel subunit Kv11.1 |
| Molecular Weight(MW) | 127 kDa(Observed band size: 90,150 kDa) |
| Cellular Localization | Cell membrane>Multi-pass membrane protein. |
WB
Western blot analysis of lysates from COLO205 cells(LPS,4h treatment), using KCNH2 Ab at 1/1000 dilution. 5ug/NC membrane strip. Exposure for 40s with ECL Kit. Bands result from membrane strip incubation.
WB
Western blot analysis of lysates from Mouse testis, using KCNH2 Antibody at 1/1000 dilution.
IHC
Rabbit polyclonal antibody to KCNH2 at 1/100 staining human astrocytoma by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.
ICC/IF
Rabbit polyclonal antibody to KCNH2 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
