WB
Western blot analysis of various lysates using KCNH2 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer using KCNH2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma using KCNH2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using KCNH2 Rabbit pAb at dilution of 1:100 (40xlens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IF-P
Immunofluorescence analysis of Mouse kidney tissue using KCNH2 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.| Product Name | KCNH2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:100-1:500 IHC:1:100-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KCNH2 |
|---|---|
| Gene Synonyms | ERG1 HERG LQT2 SQT1 ERG-1 H-ERG HERG1 Kv11.1 |
| Gene Full Name | potassium voltage-gated channel subfamily H member 2 |
| Gene Summary | This gene encodes a component of a voltage-activated potassium channel found in cardiac muscle, nerve cells, and microglia. Four copies of this protein interact with one copy of the KCNE2 protein to form a functional potassium channel. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, May 2022] |
| Alternative Names | ERG1 HERG LQT2 SQT1 ERG-1 H-ERG HERG1 Kv11.1 KCNH2 |
| Molecular Weight(MW) | 127 kDa(Observed band size:148 kDa) |
| Cellular Localization | Cell membrane, Multi-pass membrane protein. |
WB
Western blot analysis of various lysates using KCNH2 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffinembedded Human breast cancer using KCNH2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma using KCNH2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human lung cancer using KCNH2 Rabbit pAb at dilution of 1:100 (40xlens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IF-P
Immunofluorescence analysis of Mouse kidney tissue using KCNH2 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.| Application Notes | WB:1:100-1:500 IHC:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
