ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using WBP7 antibody against Hela(1),Cos-7(2),K562(3)cell,Rat testes tissue(4) . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-WBP7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KMT2B |
|---|---|
| Gene Synonyms | 2610014H22Rik CXXC10 DYT28 HRX2 mKIAA0304 MLL1B MLL2 MLL4 MRD68 RGD1308331 TRX2 WBP-7 WBP7 |
| Gene Full Name | lysine methyltransferase 2B |
| Gene Summary | This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008] |
| Alternative Names | 3'exon32-5'exon37 member of the trx/MLL family histone-lysine N-methyltransferase 2B-like histone-lysine N-methyltransferase MLL4 KMT2D lysine (K)-specific methyltransferase 2B lysine N-methyltransferase 2B mixed lineage leukemia 2 mixed lineage leukemia gene homolog 2 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog Drosophila) 4 myeloid/lymphoid or mixed-lineage leukemia 4 myeloid/lymphoid or mixed-lineage leukemia protein 4 myeloid/lymphoid or mixed-lineage leukemia protein 4 homolog Proline-rich ligand for the WW domain of FBP11 trithorax homolog 2 trithorax homologue 2 WBP-7 WW domain binding protein 7 |
| Molecular Weight(MW) | 293kDa（observed：80kDa) |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | Q9UMN6 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using WBP7 antibody against Hela(1),Cos-7(2),K562(3)cell,Rat testes tissue(4) . 6% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
