ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using Cdc42/Rho/Rac antibody against Hela(1),B16(2),A549(3),Nih3t3(4)cell,Mouse liver tissue(5),Rat testes tissue(6) . 15% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-Cdc42/Rho/Rac antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDC42/RHOC/RAC1 |
|---|---|
| Gene Synonyms | CDC42Hs G25K TKS/ARH9 ARHC H9 RHOH9/D5Ertd559e MIG5 MRD48 p21-Rac1 Rac-1 TC-25 |
| Gene Full Name | cell division cycle 42/ras homolog family member C/Rac family small GTPase 1 |
| Gene Summary | The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]/This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]/The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| Alternative Names | Cdc42 CDC42Mm cell division cycle 42 (GTP binding protein) cell division cycle 42 homolog dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein 25kD)) dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein 25kD)) G25K GTP-binding protein growth-regulating protein GTP binding protein 25kDa GTP-binding protein GTP-binding protein G25K RP1-224A6.5 small GTP binding protein small GTP binding protein CDC42 small GTP binding protein GTPase/aplysia ras-related homolog 9 (RhoC) AR ARHC ARHC epididymis secretory sperm binding protein MGC1448 MGC61427 oncogene RHO H9 ras homolog 9 (RhoC) ras homolog gene family member C RAS-related homolog 9 Rho rho cDNA clone 9 RHO MGC1448 rhoC GTPase SIG-61 silica-induced gene 61 protein small GTP binding protein RhoC/cell migration-inducing gene 5 protein MGC111543 p21-Rac1 Rac1ins ras-like protein ras-like protein TC25 RAS-related C3 botulinum substrate 1 ras-related C3 botulinum toxin substrate ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1) ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1) rho family small GTP binding protein Rac1 small GTP binding protein Rac1 Rho-related protein |
| Molecular Weight(MW) | 21kDa/22kDa/21kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane;Cytoplasm, cytoskeleton, microtubule organizing center, centrosome;Cytoplasm, cytoskeleton, spindle;Midbody;Cell projection, dendrite;Cytoplasmic vesicle, secretory vesicle, acrosome/Cell membrane;Cleavage furrow/Cell membrane;Melanosome;Cytoplasm;Cell projection, lamellipodium;Cell projection, dendrite;Synapse;Nucleus;Cell projection, ruffle membrane |
| SwissProt ID | P60953/P08134/P63000 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using Cdc42/Rho/Rac antibody against Hela(1),B16(2),A549(3),Nih3t3(4)cell,Mouse liver tissue(5),Rat testes tissue(6) . 15% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
