ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using MUTA antibody against K562(1),B16(2),Hepg2(3),Mouse liver tissue(4),Rat kidney tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-MUTA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | MMUT |
|---|---|
| Gene Synonyms | D230010K02Rik MCM MUT |
| Gene Full Name | methylmalonyl-CoA mutase |
| Gene Summary | This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
| Alternative Names | methylmalonyl Coenzyme A mutase methylmalonyl-CoA isomerase methylmalonyl-CoA mutase c.*192delA methylmalonyl-CoA mutase c.*51C>G methylmalonyl-CoA mutase variant c.1495G>A methylmalonyl-CoA mutase variant c.2011A>G methylmalonyl-CoA mutase variant c.2150G>T methylmalonyl-CoA mutase variant c.322C>T methylmalonyl-CoA mutase variant c.613_615delGAA methylmalonyl-CoA mutase variant c.636G>A methylmalonyl-CoA mutase variant c.643G>A methylmalonyl-Coenzyme A mutase mutant methylmalonyl CoA mutase rCG43751-like |
| Molecular Weight(MW) | 83kDa |
| Source | Rabbit |
| Cellular Localization | Mitochondrion matrix;Mitochondrion;Cytoplasm |
| SwissProt ID | P22033 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using MUTA antibody against K562(1),B16(2),Hepg2(3),Mouse liver tissue(4),Rat kidney tissue(5) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
