WB
Western blot analysis of various lysates, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.ICC/IF
Immunofluorescence analysis of HepG2 cellsusing MUTA Rabbit mAb at dilutionof 1:150. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingMUTA Rabbit mAb at dilution of1:100 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.| Product Name | MUTA Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MMUT |
|---|---|
| Gene Synonyms | MCM MUT |
| Gene Full Name | methylmalonyl-CoA mutase |
| Gene Summary | This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
| Alternative Names | MCM MUT MUTA |
| Molecular Weight(MW) | 83kDa(Observed band size:83kDa) |
| Cellular Localization | Mitochondrion matrix. |
WB
Western blot analysis of various lysates, using at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of HepG2 cellsusing MUTA Rabbit mAb at dilutionof 1:150. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human colon carcinoma usingMUTA Rabbit mAb at dilution of1:100 (40x lens). High pressure antigenretrieval performed with 0.01M Citrate buffer(pH 6.0) prior to IHC staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
