ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using PGAM1 antibody against Hela(1),Cos-7(2),B16(3),Hepg2(4),THP-1(5),Nih3t3(6),Mouse liver tissue(7),Rat kidney tissue(8),Rat spleen tissue(9),Rat testes tissue(10) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-PGAM1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PGAM1 |
|---|---|
| Gene Synonyms | 2310050F24Rik HEL-S-35 Pgam-1 PGAM-B PGAMA Pgmut |
| Gene Full Name | phosphoglycerate mutase 1 |
| Gene Summary | The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
| Alternative Names | BPG-dependent PGAM 1 epididymis secretory protein Li 35 PGAM-B PGAM1 phosphoglycerate mutase 1 (brain) phosphoglycerate mutase A nonmuscle form phosphoglycerate mutase isozyme B RP11-452K12.8 similar to Homo sapiens phosphoglycerate mutase (PGAM-B) mRNA with GenBank Accession Number J04173.1 |
| Molecular Weight(MW) | 28kDa |
| Source | Rabbit |
| Cellular Localization | cytoplasm;cytosol;extracellular exosome;extracellular region;ficolin-1-rich granule lumen;membrane;secretory granule lumen;Complete GO annotation on QuickGO |
| SwissProt ID | P18669 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using PGAM1 antibody against Hela(1),Cos-7(2),B16(3),Hepg2(4),THP-1(5),Nih3t3(6),Mouse liver tissue(7),Rat kidney tissue(8),Rat spleen tissue(9),Rat testes tissue(10) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
