ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using Cytokeratin 6 antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Rat testes tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-Cytokeratin 6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KRT6A |
|---|---|
| Gene Synonyms | 2310001L23Rik 2310016L08Rik 3300001P10Rik 39.1 4732484G22Rik AEI2 BCIE BIE CARD2 CK-14 CK-16 CK-17 CK-18 CK-19 CK-1B CK-2e CK-4 CK-6A CK-6C CK-6E CK-8 CK1 CK10 CK13 CK14 CK15 CK16 CK18 CK19 CK3 CK4 CK5 CK6A CK6C CK6D CK7 CK8 CYK18 CYK4 CYK8 D130054E02Rik D15Wsu77e DDD DDD1 EBS1 EBS1A EBS1B EBS1C EBS1D EBS2 EBS2A EBS2B EBS2C EBS2D EBS2E EBS2F EBS3 EBS4 EHK EHK1 EHK2 EHK2A EHK2B EndoA EndoC EPPK FNEPPK HUMCYT2A IHL K1 K10 K13 K14 K15 K16 K17 K18 K19 K1B K1C1 K1CO K1CP K1CS K1emb K2C7 K2C8 K2e K3 K4 K5 K6[a] K6A K6C K6D K7 K76 K77 K8 KO KPP Krt-1.10 Krt-1.13 Krt-1.14 Krt-1.19 Krt-2.1 Krt-2.4 Krt-2.8 Krt1-10 Krt1-13 Krt1-14 Krt1-15 Krt1-16 Krt1-17 Krt1-18 Krt1-19 KRT16A KRT1A KRT1B Krt2-1 Krt2-17 Krt2-2 Krt2-4 Krt2-5 Krt2-6a Krt2-6c Krt2-7 Krt2-8 Krt239 KRT2A KRT2B KRT2E KRT2P KRT5A KRT6C KRT6D Krt86 KRTE MECD2 mK1b mK6[a] MK6a NEPPK NFJ PC1 PC2 PC3 PCHC1 SCL Tfip8 TROMA-1 WSN1 WSN2 |
| Gene Full Name | keratin 6A |
| Gene Summary | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014] |
| Alternative Names | 40-kDa keratin intermediate filament 47 kDa cytokeratin 56 kDa cytokeratin 57kd keratin 57kDa keratin 58 kDa cytokeratin 60-kDa keratin 65 kDa cytokeratin 67 kDa cytokeratin 70 kd type II cell proliferation-inducing gene 46 protein CK-1 CK-10 CK-13 CK-15 CK-17 CK-19 CK-1B CK-2e CK-2P CK-3 CK-4 CK-5 CK-7 CK-8 cytokeratin 1 cytokeratin 10 cytokeratin 13 cytokeratin 14 cytokeratin 15 cytokeratin 16 cytokeratin 18 cytokeratin 19 cytokeratin 2 cytokeratin 3 cytokeratin 4 cytokeratin 5 cytokeratin 6A cytokeratin 6C cytokeratin 6D Cytokeratin 7 cytokeratin 8 cytokeratin endo A cytokeratin endo B cytokeratin-1 cytokeratin-10 cytokeratin-15 cytokeratin-16 cytokeratin-17 cytokeratin-18 cytokeratin-1B cytokeratin-2e cytokeratin-2P cytokeratin-4 cytokeratin-5 cytokeratin-6A cytokeratin-7 cytokeratin-8 cytoplasmic intermediate filament protein cytoskeletal 57 kDa keratin cytoskeletal protein embryonic type II keratin 1 EndoA cytokeratin (5' end put.) putative EndoA' cytokeratin (5' end put.) putative epidermal keratin 10 epidermal keratin type I epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types epidermolytic hyperkeratosis 1 epithelial keratin-2e focal non-epidermolytic palmoplantar keratoderma hair alpha protein HLA-DRB1-restricted epitope S1 HLA-DRB1-restricted epitope S2 HLA-DRB1-restricted epitope S3 HLA-DRB1-restricted epitope S4 HMWCK intermediate filament protein intermediate filament protein K16 intermediate filament protein K7 K1 K1B K2e K2P K6-alpha K76 K77 keratin 1 type II keratin 10 type I keratin 13 type I keratin 14 type I keratin 15 type I keratin 16 homolog keratin 16 type I keratin 17 type I keratin 18 type I keratin 19 type I Keratin 1B keratin 2 epidermis keratin 2 type II keratin 2p keratin 3 type II keratin 4 type II keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types) keratin 5 type II keratin 6A type II keratin 6A type II keratin 7 type II keratin 76 type II keratin 77 type II keratin 8 type II keratin 86 keratin complex 1 acidic gene 10 keratin complex 1 acidic gene 13 keratin complex 1 acidic gene 14 keratin complex 1 acidic gene 15 keratin complex 1 acidic gene 16 keratin complex 1 acidic gene 17 keratin complex 1 acidic gene 18 keratin complex 1 acidic gene 19 keratin complex 2 basic gene 1 keratin complex 2 basic gene 17 keratin complex 2 basic gene 39 keratin complex 2 basic gene 4 keratin complex 2 basic gene 5 keratin complex 2 basic gene 6a keratin complex 2 basic gene 6c keratin complex 2 basic gene 7 keratin complex 2 basic gene 8 keratin complex 2 gene 17 keratin D keratin intermediate filament 16a keratin intermediate filament 16b keratin K5 keratin type II Keratin type II cytoskeletal 5 keratin 55K type II cytoskeletal keratin epidermal type II K6A keratin simple epithelial type I K7 keratin type I cytoskeletal 59 kDa keratin type I 40-kd keratin-10 keratin-15 basic keratin-15 beta keratin-2 epidermis keratin-2e keratin-6-alpha KRT1B KRT5 mK6-alpha ORF Pan CK pan Cytokeratin pan keratin pankeratin PIG46 sarcolectin suprabasal cytokeratin 10 tuftelin interacting protein 8 type I cytoskeletal 15 type II cytoskeletal 1b type II keratin Kb39 type II keratin subunit protein type II mesothelial keratin K7 type-II intermediate filament protein type-II keratin Kb1 type-II keratin Kb2 type-II keratin Kb3 Type-II Keratin Kb39 type-II keratin Kb4 type-II keratin Kb5 type-II keratin Kb6 type-II keratin Kb7 type-II keratin Kb8 type-II keratin Kb9 |
| Molecular Weight(MW) | 60kDa（observed：55kDa) |
| Source | Rabbit |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P02538 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using Cytokeratin 6 antibody against Hela(1),Cos-7(2),K562(3),B16(4),Hepg2(5),U251(6),A549(7),Rat testes tissue(8) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
