ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using SHMT2/SHMT antibody against Hela(1),Cos-7(2),B16(3),A549(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-SHMT2 / SHMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SHMT2 |
|---|---|
| Gene Synonyms | 2700043D08Rik GLYA HEL-S-51e mSHMT NEDCASB SHMT |
| Gene Full Name | serine hydroxymethyltransferase 2 |
| Gene Summary | This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Alternative Names | epididymis secretory sperm binding protein Li 51e GLY A+ glycine auxotroph A human complement for hamster glycine hydroxymethyltransferase mitochondrial serine hydroxymethyltransferase serine aldolase serine hydroxymethylase serine hydroxymethyltransferase 2 (mitochondrial) serine hydroxymethyltransferase 2 (mitochondrial) isoform SHMT2_1 serine hydroxymethyltransferase 2 (mitochondrial) isoform SHMT2_2 serine methylase SHMT 2 threonine aldolase |
| Molecular Weight(MW) | 55kDa（observed：50kDa) |
| Source | Rabbit |
| Cellular Localization | Mitochondrion;Mitochondrion matrix, mitochondrion nucleoid;Mitochondrion inner membrane;Cytoplasm;Nucleus |
| SwissProt ID | P34897 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using SHMT2/SHMT antibody against Hela(1),Cos-7(2),B16(3),A549(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
