ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using KAT2A antibody against Huvec(1),Mcf-7(2),A431(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-KAT2A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KAT2A |
|---|---|
| Gene Synonyms | 1110051E14Rik GCN5 GCN5L2 hGCN5 mmGCN5 PCAF-b |
| Gene Full Name | lysine acetyltransferase 2A |
| Gene Summary | KAT2A, or GCN5, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. It also functions as a repressor of NF-kappa-B (see MIM 164011) by promoting ubiquitination of the NF-kappa-B subunit RELA (MIM 164014) in a HAT-independent manner (Mao et al., 2009 [PubMed 19339690]).[supplied by OMIM, Sep 2009] |
| Alternative Names | GCN5 (general control of amino-acid synthesis yeast homolog)-like 2 GCN5 general control of amino acid synthesis-like 2 general control of amino acid synthesis 5-like 2 general control of amino acid synthesis protein 5-like 2 general control of amino acid synthesis yeast homolog-like 2 General control of amino acid synthesis yeast homolog-like 2 general control of amino acid synthesis-like 2 histone acetyltransferase GCN5 histone acetyltransferase GCN5 family histone glutaryltransferase KAT2A histone succinyltransferase KAT2A hsGCN5 K(lysine) acetyltransferase 2A KATA2 lysine acetyltransferase 2A PCAF-B/GCN5 protein lactyltransferase KAT2A STAF97 |
| Molecular Weight(MW) | 93kDa |
| Source | Rabbit |
| Cellular Localization | Nucleus;Chromosome;Cytoplasm, cytoskeleton, microtubule organizing center, centrosome |
| SwissProt ID | Q92830 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using KAT2A antibody against Huvec(1),Mcf-7(2),A431(3) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | ELISA:1:1000-1:10000 WB:1:1000-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
