ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-lats1/2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LATS1/LATS2 |
|---|---|
| Gene Synonyms | WARTS wts/KPM |
| Gene Full Name | large tumor suppressor kinase 1/large tumor suppressor kinase 2 |
| Gene Summary | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017]./This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008] |
| Alternative Names | h-warts large tumor suppressor homolog 1 LATS (large tumor suppressor Drosophila) homolog 1 LATS large tumor suppressor homolog 1 putative serine threonine kinase similar to Drosophila warts tumor suppressor WARTS protein kinase./h-warts kinase phosphorylated during mitosis protein large tumor suppressor homolog 2 LATS (large tumor suppressor Drosophila) homolog 2 LATS large tumor suppressor homolog 2 serine/threonine kinase KPM serine/threonine-protein kinase kpm warts-like kinase |
| Molecular Weight(MW) | 126kDa/120kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome;Cytoplasm, cytoskeleton, spindle;Midbody;Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body./Cytoplasm, cytoskeleton, microtubule organizing center, centrosome;Cytoplasm;Cytoplasm, cytoskeleton, spindle pole;Nucleus |
| SwissProt ID | O95835|Q9NRM7 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300. |
