WB
Western blot analysis of lysates from EC304 cells, using Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/1000 dilution.IHC
Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.IHC
Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Product Name | Rabbit polyclonal antibody to Phospho-LATS1/2 (Ser909/Ser872) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human LATS1/2 around the phosphorylation site of Ser909/872. |
| Modification | p-Ser909/Ser872 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LATS1/LATS2 |
|---|---|
| Gene Synonyms | wts WARTS KPM |
| Gene Full Name | large tumor suppressor kinase 1/2 |
| Gene Summary | The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. [provided by RefSeq, Apr 2017] |
| Alternative Names | h-warts Large tumor suppressor homolog 1 LATS large tumor suppressor homolog 1 LATS1 LATS1_HUMAN Serine threonine protein kinase LATS1 Serine/threonine-protein kinase LATS1 WARTS WARTS protein kinase wts FLJ13161 Kinase phosphorylated during mitosis protein KPM Large tumor suppressor homolog 2 Large tumor suppressor homolog 2 Drosophila Large tumor suppressor kinase 2 LATS large tumor suppressor Drosophila homolog 2 LATS large tumor suppressor homolog 2 Lats2 LATS2_HUMAN Serine/threonine kinase kpm Serine/threonine protein kinase kpm Serine/threonine-protein kinase kpm Serine/threonine-protein kinase LATS2 Warts like kinase Warts-like kinase |
| Molecular Weight(MW) | 126 kDa |
| Cellular Localization | Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. |
WB
Western blot analysis of lysates from EC304 cells, using Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/1000 dilution.
IHC
Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
IHC
Phospho-LATS1/2 (Ser909/Ser872) Antibody at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
