WB
Sample loading: 2 ug total protein of Arabidopsis/rice leaves,
Electrophoresis: 12.5% SDS-PAGE,
Membrane transfer: NC (nitrocellulose) membrane, voltage 10 V overnight,
Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour,
Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour.