WB
Loading:Gradient-diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-AGO10/ Protein argonaute 10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT5G43810，Q9XGW1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | AGO10 |
|---|---|
| Gene Synonyms | AGO10, ARGONAUTE 10, PINHEAD, PNH, ZLL, ZWILLE |
| Gene Full Name | Protein argonaute 10 |
| Gene Summary | AGO10 encodes Argonaute10, a member of the EIF2C (elongation initiation factor 2c)/ Argonaute class of proteins. It is required to establish the central-peripheral organization of the embryo apex. AGO10 specifically sequesters miR166/165 to regulate shoot apical meristem development. |
| Molecular Weight(MW) | 111 kDa |
WB
Loading:Gradient-diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
