WB
Loading:Approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-ATPF / ATP synthase subunit b |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana ATCG00130. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | ATPF |
|---|---|
| Gene Synonyms | AtpF, ATP synthase F(0) sector subunit b, CF0I. |
| Gene Full Name | ATPF / ATP synthase subunit b |
| Gene Summary | F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Component of the F0 channel, it forms part of the peripheral stalk, linking F1 to F0. |
| Molecular Weight(MW) | 21 / 16 kDa |
WB
Loading:Approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
