WB
Loading:2μg Arabidopsis/rice leaf total protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-C4H/ Trans-cinnamate 4-monooxygenase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT2G30490. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | C4H |
|---|---|
| Gene Synonyms | ATC4H, C4H, cinnamate 4-monooxygenase, CYP73A5, REF3 |
| Gene Full Name | C4H/ Trans-cinnamate 4-monooxygenase |
| Gene Summary | Catalyzes the first oxidative step of the phenylpropanoid pathway in higher plants by transforming trans-cinnamate into p-coumarate. The compounds formed by this pathway are essential components for lignification, pollination, and defense against ultraviolet light, predators and pathogens |
| Molecular Weight(MW) | 58 kDa |
WB
Loading:2μg Arabidopsis/rice leaf total protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
