WB
Loading:Approximately 2 μg of total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-CHLI1 / Magnesium-chelatase subunit ChlI-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Arabidopsis thaliana AT4G18480. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | CHLI1 |
|---|---|
| Gene Synonyms | CHLI, Magnesium-chelatase subunit ChlI |
| Gene Full Name | CHLI1 / Magnesium-chelatase subunit ChlI-1 |
| Gene Summary | Magnesium-chelatase subunit ChLI is involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg-protoporphyrin IX. The reaction takes place in two steps, with an ATP-dependent activation followed by an ATP-dependent chelation step. Possesses low affinity for ATP and may play a limited role in chlorophyll biosynthesis, and contributes to the assembly of the Mg-chelatase complex. |
| Molecular Weight(MW) | 46 kDa |
WB
Loading:Approximately 2 μg of total protein from Arabidopsis leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
