WB
Loading:1 0.1ug E.coli lysate protein (containing recombinant Cas9-expressed protein); 2 2ng E.coli lysate protein; 3 1ng E.coli lysate protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-Anti-Cas9/ CRISPR-associated endonuclease |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | Cas9/CRISPR |
|---|---|
| Gene Synonyms | SpCas9，SpyCas9，csn1 |
| Gene Full Name | Cas9/ CRISPR-associated endonuclease |
| Gene Summary | CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements. |
| Molecular Weight(MW) | 47kDa |
WB
Loading:1 0.1ug E.coli lysate protein (containing recombinant Cas9-expressed protein); 2 2ng E.coli lysate protein; 3 1ng E.coli lysate protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
