WB
Loading: serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-CYP74A/AOS/ Allene oxide synthase, chloroplastic |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT5G42650，Q96242 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | CYP74A |
|---|---|
| Gene Synonyms | ALLENE OXIDE SYNTHASE AOS CYP74A CYTOCHROME P450 74A DDE2 DELAYED DEHISCENCE 2，Cytochrome P450 74A，Hydroperoxide dehydrase |
| Gene Full Name | CYP74A/AOS/ Allene oxide synthase, chloroplastic |
| Gene Summary | AOS encodes a member of the cytochrome p450 CYP74 gene family that functions as an allene oxide synthase. This enzyme catalyzes dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway. |
| Molecular Weight(MW) | 58.2 / 54.6kDa |
WB
Loading: serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
