WB
Loading:serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-DMT1/ DNA (cytosine-5)-methyltransferase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated peptide from Arabidopsis thaliana AT5G49160，P34881 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | DMT1 |
|---|---|
| Gene Synonyms | ATHIM, DDM2, DMT01, MET1, MET2，DMT1 |
| Gene Full Name | DMT1/ DNA (cytosine-5)-methyltransferase 1 |
| Gene Summary | MET1 encodes a cytosine methyltransferase. It maintains chromatin CpG methylation that plays a role in genomic imprinting, regulation of embryogenesis and seed viability. It is required for proper patterns of CG DNA methylation in dividing cells, and for MEA promoter methylation in seeds. |
| Molecular Weight(MW) | 172.4 kDa |
WB
Loading:serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
