WB
Loading:approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-DWF1/ Delta(24)-sterol reductase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT3G19820 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | DWF1 |
|---|---|
| Gene Synonyms | CABBAGE 1, CBB1, DIM, DIM1, DIMINUTIA, DIMINUTO 1, DWARF 1, DWF1, ENHANCED VERY-LOW-FLUENCE RESPONSES 1, EVE1 |
| Gene Full Name | DWF1/ Delta(24)-sterol reductase |
| Gene Summary | DWF1 is a Ca2+-dependent calmodulin-binding protein. It is involved in the conversion of the early brassinosteroid precursor 24-methylenecholesterol to campesterol. Brassinosteroids affect cellular elongation. |
| Molecular Weight(MW) | 65.4 kDa |
WB
Loading:approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
