WB
Loading:serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-ECHID/ Enoyl-CoA hydratase/isomerase D |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G60550，Q8GYN9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | ECHID |
|---|---|
| Gene Synonyms | DHNS, ECHID, ENOYL-COA HYDRATASE/ISOMERASE D，1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal |
| Gene Full Name | ECHID/ Enoyl-CoA hydratase/isomerase D |
| Gene Summary | ECHID is involved in the biosynthesis of phylloquinone (vitamin K1). It converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA). It is essential for chloroplast development in Arabidopsis |
| Molecular Weight(MW) | 37 kDa |
WB
Loading:serially diluted antigen protein Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
