WB
Loading:approximately 3 μg of total protein from Arabidopsis thaliana Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-ERF-3/ Polypeptide release factor 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis thaliana AT1G18070，Q8L835 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:10000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1μg/μl |
| Purification | Affinity purified |
| Gene Symbol | ERF-3 |
|---|---|
| Gene Synonyms | EF-1ALPHA，ERF-3，ERF2，Translation release factor 3，Eukaryotic peptide chain release factor GTP-binding subunit |
| Gene Full Name | ERF-3/ Polypeptide release factor 3 |
| Gene Summary | Translation elongation factor EF1A/initiation factor IF2gamma family protein. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis |
| Molecular Weight(MW) | 59.2 kDa |
WB
Loading:approximately 3 μg of total protein from Arabidopsis thaliana Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
