WB
Loading:Approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Product Name | Anti-GUN5/CHLH/MgCh/Magnesium chelatase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein of Rice LOC_Os03g20700 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | GUN5 ABA-BINDING PROTEIN ABAR CCH CCH1 CHLH H SUBUNIT OF MG-CHELATASE |
|---|---|
| Molecular Weight(MW) | 153/150 kDakDa |
| Function | GUN5 is a plastid-localized Mg-chelatase enzyme subunit (CHLH), and it is a multifunctional protein involved in tetrapyrrole biosynthesis, plastid signaling and ABA responses in guard cells. |
WB
Loading:Approximately 2 μg of total protein from Arabidopsis thaliana and rice leaves Electrophoresis: 12% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, 10V overnight Blocking: 5% skim milk, incubation at room temperature for 1h Primary antibody: 1:5000 dilution, incubation at room temperature for 1h| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
