WB
Sample loading: Gradient diluted antigen protein, electrophoresis: 10% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, room temperature incubation for 1 hour. Primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.| Product Name | Anti-HCAR/ 7-hydroxymethyl chlorophyll a reductase |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant part protein of Arabidopsis AT1G04620, Q8GS60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500-1:5000 |
|
| Species Reactivity | ArabidopsisRice |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | 7-HYDROXYMETHYL CHLOROPHYLL A (HMCHL) REDUCTASE HCAR |
|---|---|
| Molecular Weight(MW) | 52 / 49 kDa for Arabidopsis |
| Function | HCAR encodes a 7-hydroxymethyl chlorophyll a reductase, an enzyme of the chlorophyll cycle that converts 7-hydroxymethyl chlorophyll a to chlorophyll a. It belongs to the chlorophyll catabolic enzymes (CCEs). |
WB
Sample loading: Gradient diluted antigen protein, electrophoresis: 10% SDS-PAGE, membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight, blocking: 5% skim milk powder, room temperature incubation for 1 hour. Primary antibody: 1:5000 dilution, room temperature incubation for 1 hour.| Application Notes | WB:1:2500-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 50% glycerol，-20 °C |
