WB
1-0.1 μ g E. coli lysis protein (containing Cas9 recombinant expression protein) 2-2 ng E. coli lysis protein (containing Cas9 recombinant expression protein) 3-1 ng E. coli lysis protein (containing Cas9 recombinant expression protein) electrophoresis: 12.5% SDS-PAGE. Membrane transfer: NC (nitrocellulose) membrane, 10v overnight. Closure: 5% skim milk, incubated at room temperature for 1 hour. Primary antibody: 1:5000, incubated at room temperature for 1 hour.