WB
1–0.1μg E. coli lysate (containing recombinant HPT protein) 2-2ng E. coli lysate (containing recombinant HPT protein) 3-1ng E. coli lysate (containing recombinant HPT protein) Electrophoresis: 12% SDS-PAGE. Transfer: NC (nitrocellulose) membrane, 10 V, overnight. Blocking: 5% non-fat milk, incubated for 1 hour at room temperature. Primary antibody: 1:5000, incubated for 1 hour at room temperature.| Product Name | Anti-HPT Rabbit Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length protein of Escherichia coli ABI30072，P00557 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000-1:5000 |
| Gene Symbol | ABI30072 |
|---|---|
| Alternative Names | HPT |
| Molecular Weight(MW) | 38 kDa |
| Function | The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related compounds such as demethyl analogs and destomycin. |
WB
1–0.1μg E. coli lysate (containing recombinant HPT protein) 2-2ng E. coli lysate (containing recombinant HPT protein) 3-1ng E. coli lysate (containing recombinant HPT protein) Electrophoresis: 12% SDS-PAGE. Transfer: NC (nitrocellulose) membrane, 10 V, overnight. Blocking: 5% non-fat milk, incubated for 1 hour at room temperature. Primary antibody: 1:5000, incubated for 1 hour at room temperature.| Application Notes | WB:1:1000-1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20 °C |
| Storage Buffer | 50% glycerol |
